Journal: JCI Insight

Article Title: Gut mucosal cells transfer α -synuclein to the vagus nerve

doi: 10.1172/jci.insight.172192

Figure Lengend Snippet: ( A ) Intestinal organoids were prepared from an SNCA A53T mouse in which CCK-containing cells express enhanced green fluorescent protein (eGFP), and vagal nodose ganglia neurons were isolated from an Snca –/– mouse lacking endogenous α-synuclein. ( B ) Representative images of organoids and neurons grown in coculture for 5 days, with eGFP-positive cells (green) in the organoid and β-tubulin III (Tuj1, cyan) highlighting neuronal processes. ( C ) Representative high-magnification α-synuclein (red) staining of an eGFP-positive EEC. Red arrow indicates localization to a PGP9.5-positive (cyan) process in an Snca –/– mouse neuron. ( D and E ) Representative images with neuron-specific β-tubulin III (cyan). Surface and (adjacent) intracellular confocal slices are shown. Scale bars are 30 μm for B , 3 μm for C , and 5 μm for D and E .

Article Snippet: Primary antibodies used for immunostaining included rabbit CCK , rabbit α-synuclein (Abcam catalog ab138501, RRID:AB_2537217, at 1:1,000), guinea pig PGP9.5 (Abcam catalog ab10410, RRID:AB_297150, at 1:100), chick β-tubulin III (Tuj1; Neuromics catalog CH23005, RRID:AB_2210684, at 1:100), and chick GFP (Abcam catalog ab13970, RRID:AB_300798, at 1:1,000).

Techniques: Isolation, Staining

Journal:

Article Title: Unmyelinated Auditory Type I Spiral Ganglion Neurons in Congenic Ly5.1 Mice

doi: 10.1002/cne.22398

Figure Lengend Snippet: Antibody Characterization

Article Snippet: Class III β-Tubulin , Antibody raised against microtubules derived from rat brain , Rabbit , MRB435p , Covance , 1:200.

Techniques: Derivative Assay

Journal:

Article Title: Unmyelinated Auditory Type I Spiral Ganglion Neurons in Congenic Ly5.1 Mice

doi: 10.1002/cne.22398

Figure Lengend Snippet: A–C. Dual labeling with anti-CNPase (green) and anti-TuJ1 III β-tubulin (red) in a CBA/CaJ mouse. TuJ1 antibody preferentially labels the cytoplasm of large type I neurons (Sekerkova et al., 2008). CNPase labeling of Schwann cells and their myelin sheaths results in a honeycomb-like pattern in the SG. D–F. Dual labeling with anti-CNPase (green) and anti-TuJ1 III β-tubulin (red) in an Ly5.1 mouse. The honeycomb-like pattern is completely disrupted in the apical turn. Most SGNs lacking a CNPase-positive myelin sheath stained positively for TuJ1. G–I. Dual labeling with anti-Na, K-ATPase (green) and anti-neurofilament 200 (NF 200, red) in a CBA/CaJ mouse. Strong surface staining for Na, K-ATPase is seen in most of the SGNs J–L. Dual labeling with anti-Na, K-ATPase (green) and anti-neurofilament 200 (NF 200, red) in an Ly5.1 mouse. Again, most NF200-positive neurons were co-labeled with Na, K-ATPase. Scale bar: A–L, 25 µm. A magenta-green copy of this figure is available as Supplementary Figure 3.

Article Snippet: Class III β-Tubulin , Antibody raised against microtubules derived from rat brain , Rabbit , MRB435p , Covance , 1:200.

Techniques: Labeling, Staining

Journal: Acta Naturae

Article Title: Morphological Characterization of Astrocytes in a Xenograft of Human iPSCDerived Neural Precursor Cells

doi: 10.32607/actanaturae.11710

Figure Lengend Snippet: Neural markers in the IPSC culture and in the graft. ( A ) Beta-3-tubilin in culture (TUBB3, red). ( B ) Human neuron-specific enolase (NSE) in the graft area (immunoperoxidase staining). ( C ) Human tyrosine hydroxylase-positive neurons in the graft (HNA, green; TH, red). Scale bar: ( A ), ( C ), 50 μm

Article Snippet: The cell culture was stained for beta-3-tubulin (anti-TUJ1 antibodies, Nordic Biosite, Sweden) to detect neural progenitors.

Techniques: Immunoperoxidase Staining