Journal:

Article Title: Unmyelinated Auditory Type I Spiral Ganglion Neurons in Congenic Ly5.1 Mice

doi: 10.1002/cne.22398

Figure Lengend Snippet: Antibody Characterization

Article Snippet: Class III β-Tubulin , Antibody raised against microtubules derived from rat brain , Rabbit , MRB435p , Covance , 1:200.

Techniques: Derivative Assay

Journal:

Article Title: Unmyelinated Auditory Type I Spiral Ganglion Neurons in Congenic Ly5.1 Mice

doi: 10.1002/cne.22398

Figure Lengend Snippet: A–C. Dual labeling with anti-CNPase (green) and anti-TuJ1 III β-tubulin (red) in a CBA/CaJ mouse. TuJ1 antibody preferentially labels the cytoplasm of large type I neurons (Sekerkova et al., 2008). CNPase labeling of Schwann cells and their myelin sheaths results in a honeycomb-like pattern in the SG. D–F. Dual labeling with anti-CNPase (green) and anti-TuJ1 III β-tubulin (red) in an Ly5.1 mouse. The honeycomb-like pattern is completely disrupted in the apical turn. Most SGNs lacking a CNPase-positive myelin sheath stained positively for TuJ1. G–I. Dual labeling with anti-Na, K-ATPase (green) and anti-neurofilament 200 (NF 200, red) in a CBA/CaJ mouse. Strong surface staining for Na, K-ATPase is seen in most of the SGNs J–L. Dual labeling with anti-Na, K-ATPase (green) and anti-neurofilament 200 (NF 200, red) in an Ly5.1 mouse. Again, most NF200-positive neurons were co-labeled with Na, K-ATPase. Scale bar: A–L, 25 µm. A magenta-green copy of this figure is available as Supplementary Figure 3.

Article Snippet: Class III β-Tubulin , Antibody raised against microtubules derived from rat brain , Rabbit , MRB435p , Covance , 1:200.

Techniques: Labeling, Staining

Journal: Scientific Reports

Article Title: IL-10 Promotes Neurite Outgrowth and Synapse Formation in Cultured Cortical Neurons after the Oxygen-Glucose Deprivation via JAK1/STAT3 Pathway

doi: 10.1038/srep30459

Figure Lengend Snippet: ( A ) At day 2, neurons were small with relatively round cell bodies and short neurites (×200). ( B ) At day 7, the neurites of neurons extended and formed a network (×200). ( C ) Immunofluorescence staining of cultured neurons. Cell bodies and neurites were stained with class III-β-Tubulin (red), while nuclei were labeled with Hoechst33342 (blue). Scale bar = 50 μm.

Article Snippet: Cells were incubated overnight at 4 °C with the following primary antibodies: rabbit anti-IL-10R1 antibody (1:300, Santa Cruz, USA), rabbit anti-Netrin-1 antibody (1:200, Abcam, UK), rabbit anti-GFP antibody (1:1000, Abcam, UK), mouse anti-vGLUT antibody (1:200, Abcam, UK), anti-vGAT antibody (1:50, Santa Cruz, USA) and mouse anti-class III-β-Tubulin antibody (1:400, Beyotime, China).

Techniques: Immunofluorescence, Staining, Cell Culture, Labeling

Journal: Scientific Reports

Article Title: IL-10 Promotes Neurite Outgrowth and Synapse Formation in Cultured Cortical Neurons after the Oxygen-Glucose Deprivation via JAK1/STAT3 Pathway

doi: 10.1038/srep30459

Figure Lengend Snippet: ( A ) qPCR analysis of IL-10R1 (n = 3). Control group was set as calibrator sample representing the 1 × expression. *** p < 0.001, as compared with the control group; by unpaired two-tailed Student’s t-tests. Data are presented as mean ± SEM. ( B ) Western blot analysis of IL-10R1 (n = 5). *** p < 0.001, as compared with the control group; by unpaired two-tailed Student’s t-tests. Data are presented as mean ± SEM. ( C ) Localization of immunoreactive IL-10R1 expression on neurons before and after OGD. The left column displays neuronal marker class III-β-Tubulin (red) and nucleus (blue). The middle column shows expression of IL-10R1 (green), which was expressed in the cyton and neurites. The right column shows the co-localization of IL-10R1 and class III-β-Tubulin (yellow). Scale bar represents 20 μm.

Article Snippet: Cells were incubated overnight at 4 °C with the following primary antibodies: rabbit anti-IL-10R1 antibody (1:300, Santa Cruz, USA), rabbit anti-Netrin-1 antibody (1:200, Abcam, UK), rabbit anti-GFP antibody (1:1000, Abcam, UK), mouse anti-vGLUT antibody (1:200, Abcam, UK), anti-vGAT antibody (1:50, Santa Cruz, USA) and mouse anti-class III-β-Tubulin antibody (1:400, Beyotime, China).

Techniques: Control, Expressing, Two Tailed Test, Western Blot, Marker

Journal: Scientific Reports

Article Title: IL-10 Promotes Neurite Outgrowth and Synapse Formation in Cultured Cortical Neurons after the Oxygen-Glucose Deprivation via JAK1/STAT3 Pathway

doi: 10.1038/srep30459

Figure Lengend Snippet: Representative images of each group were shown. The left column displays neuronal marker class III-β-Tubulin (red) and nucleus (blue). The length of the longest neurites and the number of primary neurites were counted. The middle column shows expression of Netrin-1 (green), which was expressed in the cyton and neurites. The right column shows the co-localization of Netrin-1 and class III-β-Tubulin (yellow). Scale bar represents 20 μm.

Article Snippet: Cells were incubated overnight at 4 °C with the following primary antibodies: rabbit anti-IL-10R1 antibody (1:300, Santa Cruz, USA), rabbit anti-Netrin-1 antibody (1:200, Abcam, UK), rabbit anti-GFP antibody (1:1000, Abcam, UK), mouse anti-vGLUT antibody (1:200, Abcam, UK), anti-vGAT antibody (1:50, Santa Cruz, USA) and mouse anti-class III-β-Tubulin antibody (1:400, Beyotime, China).

Techniques: Marker, Expressing

Journal: Acta Naturae

Article Title: Morphological Characterization of Astrocytes in a Xenograft of Human iPSCDerived Neural Precursor Cells

doi: 10.32607/actanaturae.11710

Figure Lengend Snippet: Neural markers in the IPSC culture and in the graft. ( A ) Beta-3-tubilin in culture (TUBB3, red). ( B ) Human neuron-specific enolase (NSE) in the graft area (immunoperoxidase staining). ( C ) Human tyrosine hydroxylase-positive neurons in the graft (HNA, green; TH, red). Scale bar: ( A ), ( C ), 50 μm

Article Snippet: The cell culture was stained for beta-3-tubulin (anti-TUJ1 antibodies, Nordic Biosite, Sweden) to detect neural progenitors.

Techniques: Immunoperoxidase Staining